CF®640R TUNEL Assay Apoptosis Detection Kit from Biotium, Inc.

Product Specs
ItemCF®640R TUNEL Assay Apoptosis Detection Kit
CompanyBiotium, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Number30074
Source/Expression SystemBSA from bovine serum (cow; Bos taurus); TdT enzyme (calf thymus; recombinant, produced in E. coli)

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Biotium, Inc.

46117 Landing Parkway

Fremont, CA 94538

United States

Phone: 510-265-1027
510-265-1352

Email: order@biotium.com

Company Profile

Website: https://biotium.com/company/

(1) Topical application of synthetic melanin promotes tissue repairnpj Regenerative MedicineNovember 2, 2023Dauren Biyashev, Zofia E. Siwicka, Ummiye V. Onay, Michael Demczuk, Dan Xu, Madison K. Ernst, Spencer T. Evans, Cuong V. Nguyen, Florencia A. Son, Navjit K. Paul, et al.The TUNEL assay was performed using the CF®640 R Dye TUNEL assay apoptosis detection kits (Biotium, 30074) according to the manufacturer’s directions and visualized with an ECLIPSE Ti2 inverted microscope (Nikon

(2) RANKL+ senescent cells under mechanical stress: a therapeutic target for orthodontic root resorption using senolyticsInternational Journal of Oral ScienceMay 30, 2023Yue Zhou, Aki Nishiura, Hidetoshi Morikuni, Wenqi Deng, Toru Tsujibayashi, Yoshihiro Momota, Yuki Azetsu, Masamichi Takami, Yoshitomo Honda, Naoyuki MatsumotoTUNEL staining reagents were obtained from the CF™ Dye TUNEL Assay Kits ALEXA FLUOR® 647 (Cat. No. 30074, Fermont, CA, Biotium, Inc.). All sections were stained according to the protocol in kit and mounted

(3) Reduced calcium levels and accumulation of abnormal insulin granules in stem cell models of HNF1A deficiencyCommunications BiologyDecember 1, 2022Bryan J. González et al.3% donkey serum with 0.1% triton X-100 diluted in PBS overnight at 4 °C. For tunel assay (Biotium, 30074) cells were incubated with fluorescent biotinylated nucleotide conjugate according to the manufacturer’s

(4) Lacosamide decreases neonatal seizures without increasing apoptosisEpilepsiaDecember 1, 2022Rachel L. Langton, Shaunik Sharma, Grant C. Tiarks, Alexander G. Bassuk, Joseph Glykyswere labeled with deoxyuridine triphosphate conjugated to a photostable far‐red fluorescent dye (Cat‐30074, Biotium) using the TUNEL method according to Biotium's commercial protocol. We used a TUNEL+ control

(5) Disrupting autorepression circuitry generates “open-loop lethality” to yield escape-resistant antiviral agentsCellJune 9, 2022Sonali Chaturvedi et al.Detection Kit, Biolegend, Cat # 640932) or by using the TUNEL Assay Apoptosis Detection kit (Biotium, Cat #30074) according to the manufacturer’s instructions. TLR9 expression analysis was performed on ARPE-19 cells

(6) The AIM2 inflammasome is activated in astrocytes during the late phase of EAEJCI InsightApril 22, 2022William E. Barclay, Nupur Aggarwal, M. Elizabeth Deerhake, Makoto Inoue, Toshiaki Nonaka, Kengo Nozaki, Nathan A. Luzum, Edward A. Miao, Mari L. Shinoharathick floating tissue sections with the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, catalog 30074). Antibodies used for staining are indicated in Supplemental Table 1. Preparation of tissue slice culture

(7) Secreted osteopontin from CD4⁺ T cells limits acute graft-versus-host diseaseCell reportsDecember 28, 2021Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML.antibodies (listed in Key resources Table) and CF640R TUNEL (640R TUNEL Assay Apoptosis Detection Kit; 30074, Biotium) for immunofluorescence analysis. Intenstinal permeability assay using FITC-Dextran Mice

(8) Efferocytosis by Paneth cells within the intestineCurrent BiologyJune 1, 2021Laura S. Shankman, Samantha T. Fleury, W. Britt Evans, Kristen K. Penberthy, Sanja Arandjelovic, Richard S. Blumberg, Hervé Agaisse, Kodi S. Ravichandranstaining. One set of enteroids underwent apoptosis detection using the CF640R TUNEL Assay (Biotium #30074) allowing the reagent to sit for 2 hours at 37°C before EDU detection (GeneCopoeia #A003) for 30min.

(9) Potential circadian effects on translational failure for neuroprotectionNatureJune 18, 2020Elga Esposito et al.BSA blocking for 1 hr at room temperature. The slides were then transferred to the TUNEL (Biotium, 30074) working solution for 1 hr at 37 °C and then rinsed. To combine with Fluoro-Jade, the slides were then

(10) Irradiation abolishes smooth muscle investment into vascular lesions in specific vascular bedsJCI InsightAugust 9, 2018Alexandra A.C. Newman, Richard A. Baylis, Daniel L. Hess, Steven D. Griffith, Laura S. Shankman, Olga A. Cherepanova, Gary K. Owenscontrol. TUNEL staining was performed using the CF 640R TUNEL Assay Apoptosis Detection Kit (Biotium, 30074; lot 180417). Cells were counter stained with DAPI (Thermo Fisher Scientific, D3571). Secondary antibodies

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